DOI: 10.5937/jaes18-26895
This is an open access article distributed under the CC BY 4.0
Volume 18 article 707 pages: 413 - 421
In this study concentration of toxic elements As, Cd, and Pb were determined in different soil types and belonging
orchid species Anacamptis morio vital parts, in order to examine accumulation patterns and provide new insights
about the potential use of this orchid in bioremediation technology. Soils developed on limestone, serpentine, and
the chert were subjected to the BCR sequential extraction. Samples of orchid roots and tubers, as underground
parts, and stems, leaves, and inflorescences, as above-ground organs, were also analyzed for the content of As,
Cd and Pb. During this research, it was observed that metal content in soil is directly proportional to its content in
the plant, more specifically in roots, which suggests that A. morio can potentially be used in the phytostabilization of
contaminated sites. Values for BCF factors showed Cd immobilization in roots regardless of the soil type. A certain
level of arsenic was transferred from roots to leaves indicating the potential for accumulation of this element into aboveground organs. Assessment of the phytoremediation potential of this orchid or another plant species from diverse
environments is important as it provides information about the possibility of their future application in environmental
remediation programs.
This research was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grants No. 451-03-68/2020-14/200023, 451-03-68/2020-14/ 200178, 451–03–68/2020–14/200007 and 451-03-68/2020-14/200168).
1. Dixit, R., Malaviya, D., Pandiyan , K., Singh, B., Sahu, A., Shukla, R., Singh, P., Rai, J., Sharma, P., Lade, H., Paul, D., (2015), Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes, Sustainability, 7(2), 2189-2212, DOI: 10.3390/su7022189
2. Banuelos, G., Ajwa, H., (2008), Trace elements in soils and plants: An overview, Toxic/Hazardous Substances, and Environmental Engineering, 34, 951- 974., DOI: 10.1080/10934529909376875
3. Bakircioglu, D., Kurtulus, Y., Ibar, H., (2011), Investigation of trace elements in agricultural soils by BCR sequential extraction method and its transfer to wheat plants, Environmental Monitoring and Assessment, 175, 303–314. DOI: 10.1007/s10661- 010-1513-5
4. Mani, D., Kumar, C., (2014), Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation, International Journal of Environmental Science and Technology, 11, 843– 872., DOI: 10.1007/s13762-013-0299-8
5. Ali, H., Khan, E., Sajad, M., (2013), Phytoremediation of heavy metals—Concepts and applications, Chemosphere, 91, 869-881., DOI: 10.1016/j.chemosphere.2013.01.075]
6. Lasat, M., (2002), Phytoextraction of Toxic Metals: A Review of Biological Mechanisms, Journal of Environmental Quality, 31 (1), 109, DOI: 10.2134/ jeq2002.1090
7. Mendez, M., Maier, R., (2008), Phytostabilization of Mine Tailings in Arid and Semiarid Environments— An Emerging Remediation Technology, Environmental Health Perspectives, volume 116(3), 278-283, DOI: 10.1289/ehp.10608
8. Tica, D., Udovic, M., Lestan, D., (2011), Immobilization of potentially toxic metals using different soil amendments, Chemosphere, 85, 577-583., DOI: 10.1016/j.chemosphere.2011.06.085
9. Naidu, R., Bolan, N., Megharaj, M., Juhasz, A., Gupta, S., Clothier, B., Schulin, R., (2008), Chemical bioavailability in terrestrial environments, Developments in Soil Science, 32, 1-6, DOI: 10.1016/S0166- 2481(07)32001-1
10. Fernandez Albores, A., Perez Cid, B., Fernandez Gomez, E., Falque Lopez, E., (2000), Comparison between sequential extraction procedures and single extractions for metal partitioning in sewage sludge samples, Analyst, 125, 1353-1357, DOI: 10.1039/ B001983F
11. Ure, A., (1996), Single extraction schemes for soil analysis and related Applications, Science of The Total Environment, 178 (1-3), 3-10, DOI: 10.1016/0048- 9697(95)04791-3style="text-align: left;">12. Rao, C., Sahuquillo, A., Lopez Sanchez, J., (2008), A Review of the Different Methods Applied in Environmental Geochemistry For Single and Sequential Extraction of Trace Elements in Soils and Related Materials, Water, Air, and Soil Pollution, 189, 291– 333, DOI: 10.1007/s11270-007-9564-0
13. Tlustos, P., Szakova, A., Pavlikova, D., (2005), A comparison of sequential extraction procedures for fractionation of arsenic, cadmium, lead, and zinc in soil, Central European Journal of Chemistry, 3, 830– 851, DOI: 10.2478/BF02475207
14. De Agostini, A., Caltagironeb, C., Careddaa, A., Cicatellic, A., Cogonia, A., Farcid, D., Guarinoc, F., Garaub, A., Labrae, M., Michele Lussua, M., Dario Pianoa, D., Sannaa, C., Tommasie, N., Vaccab, A., Cortis, P., (2020), Heavy metal tolerance of orchid populations growing on abandoned mine tailings: A case study in Sardinia Island (Italy), Ecotoxicology and Environmental Safety, 189, 111018, DOI: 10.1016/j.ecoenv.2019.110018
15. Filimonova, E., Lukina, N., Glazyrina, M., Borisova, G., Maleva, M., Chukina, N., (2019), Endangered orchid plant Epipactis atrorubens on serpentine and granite outcrops of Middle Urals, Russia: A comparative morphophysiological study, AIP Conference Proceedings 2063, 040016, DOI: 10.1063/1.5087348
16. Filimonova, E., Lukina, N., Glazyrina1, M., Borisova, G., Kumar, A., Maleva, M., (2019), A comparative study of Epipactis atrorubens in two different forest communities of the Middle Urals, Russia, Journal of Forestry Research, DOI: 10.1007/s11676-019- 01010-y
17. Djordjevic, V., Tsiftsis, S., Lakusic, D., Stevanovic, V., (2016), Niche analysis of orchids of serpentine and non-serpentine areas: Implications for conservation, Plant Biosystems – An International Journal Dealing with all Aspects of Plant Biology, 150, 710- 719, DOI: 10.1080/11263504.2014.990534
18. Djordjevic, V., Tsiftsis, S., (2019), Patterns of orchid species richness and composition in relation to geological substrates, Wulfenia 26, 1–21
19. D.C., Ghosh, M., Singh, S. P. (2005), A comparative study of cadmium phytoextraction by accumulator and weed species. Environmental Pollution, 133: 365–371., DOI: 10.1016/j.envpol.2004.05.015
20. Gupta, S., Nayek, S., Saha, R.N., Satpati, S. (2008): Assessment of heavy metal accumulation in macrophyte, agricultural soil, and crop plants adjacent to discharge zone of sponge iron factory, Environmental Geology, 55, 731–739., DOI: 10.1007/s00254- 007-1025-y
21. StatSoft. (2007). Statistica for Windows, version 8.0. Tulsa: StatSoft Inc
22. Kabata-Pendias, A. (2001): Trace Elements in Soil and Plants, (2nd ed.), Boca Raton, CRC Press, Washington, D.C
23. Salminen, R., Batista, M.J., Bidovec, M., Demetriades, A., De Vivo, B., De Vos, W., Duris, M., Gilucis, A., Gregorauskiene, V., Halamic, J., et al. (2005): Geochemical atlas of Europe. Part 1: background information, methodology and maps. Espoo: Geological Survey of Finland.
24. Babalonas, D., Karataglis, S., Kabassakalis, V., (2008), Zinc, Lead and Copper Concentrations in Plants and Soils from two Mines in Chalkidiki, North Greece, Journal of Agronomy and Crop Science, volume 21, 707-713. DOI: 10.1111/j.1439-037x.1987. tb01150.x
25. Jurkiewicz, A., Turnau, K., Mesjasz-Przybylowicz, J., Przybylowicz, W., and Godzik, B., (2001), Heavy metal localization in mycorrhizas of Epipactis atrorubens (Hoffm.) Besser (Orchidaceae) from zinc mine tailings, Protoplasma, volume 218, 117-124. DOI: 10.1007/BF01306601
26. Shefferson, R., Kull, T., Tal, K., (2008), Mycorrhizal interactions of orchids colonizing Estonian mine tailings, American Journal of Botany 95(2), 156-164., DOI: 10.3732/ajb.95.2.156